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Accueil > Moyens analytiques > EA and GC-IRMS

EA and GC-IRMS

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BioGeoChemistry Lab
The laboratory of isotope biogeochemistry is fully equipped for bulk stable isotope analysis and compound specific isotope analysis of organic compounds. A Delta V plus gas source mass spectrometers in continuous flow configuration with a gas chromatograph enabling compound specific isotope analysis of 2H, 13C, and 15N. Organic chemical analysis is performed with a GC-MS to identify and quantify (semi-) volatile compounds.

1GC-MS 1GC-FID 1GC-C-IRMS 1EA

Elementar Analyser (EA) -Isotope Ratio Mass Spectrometer : %C, %N, 13C, 15N
Solid and liquid samples are flash combusted in a helium and oxygen gas flow into the first furnace of EA (1000°C). A second furnace (550°C) reduced NOx. Then the produced gas of CO2 and N2 (H2O is trapped by a Mg(ClO4)2 trap) are separated on chromatographic column and we can know the percentage of C and N in the bulk. Both gases are transported via open split (Conflo IV) in the IRMS and the isotope ratios of 13C and 15N are determined.

Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometer (GC-C-IRMS) : 13C, 15N
Organic mixtures are separated by a gas chromatography system. The separated individual compounds are online combusted (1000°C) and the generated gases of CO2 and N2 (H2O is trapped by a nafion membrane) are transported via open split (Conflo IV) into the IRMS and the isotope ratios of 13C and 15N are determined.

Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometer (GC-C-IRMS) : 2H
Organic mixtures are separated by a gas chromatography system. The separated individual compounds are online decomposed (1450°C) to produce H2 which is isotopically characterized by IRMS.
Gas Chromatography-Mass Spectrometer (GC-MS) : identification and quantification of organic compounds
Organic mixtures are separated by a gas chromatography system. The separated individual compounds are ionized by an Electron Impact (EI) to produced ions. MS detector can analyse and quantify specific m/z. An Internal Standard (IS) is used to avoid drifts.

Gas Chromatography-Flame Ionization Detector (GC-FID) : identification and quantification of organic compounds
Organic mixtures are separated by a gas chromatography system. The separated individual compounds are ionized by the flame which is surrounded by electrodes. The increase current is translated and appears as a peak in a chromatogram.

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Benoit Guyot, AI